Serotonergic 5HT7 receptor compounds for treating ocular and CNS disorders

ABSTRACT

Compounds with 5HT 7  receptor affinity (some of which are novel) useful for lowering IOP, improving blood flow to the optic nerve head and the retina, providing neuroprotection, and treating retinal diseases are disclosed. The Compounds are also useful for treating sleep disorders, depression, and other psychiatric disorders, such as, schizophrenia, anxiety, obsessive compulsive disorder, circadian rhythm disorders, and centrally and peripherally mediated hypertension. Compositions and methods for their use are also disclosed.

This application is a divisional application of U.S. Ser. No. 09/674,403, filed Oct. 31, 2000 now U.S. Pat. No. 6,960,579, which is a 371 application of PCT/US99/10179, filed May 10, 1999, which claims priority from U.S. Provisional Applications, U.S. Ser. No. 60/086,006, filed May 19, 1998, U.S. Ser. No. 60/086,005, filed May 19, 1998, U.S. Ser. No. 60/086,002, filed May 19, 1998, and U.S. Ser. No. 60/085,989, filed May 19, 1998.

The present invention is directed to the use of compounds with serotonergic 5HT₇ receptor affinity (Compound) (some of which are novel), to improve blood flow to the optic nerve head and the retina, provide neuroprotection, lower intraocular pressure (IOP), and treat retinal diseases, such as, glaucoma, age related macular degeneration (ARMD), optic neuritis, ischemic disorders, diabetic retinopathy, and retinal edema. The Compounds are also useful for treating sleep disorders, depression, and other psychiatric disorders, such as, schizophrenia, anxiety, obsessive compulsive disorder, circadian rhythm disorders, and centrally and peripherally mediated hypertension.

BACKGROUND OF THE INVENTION

Serotonin (5-hydroxy tryptamine; 5HT) is an endogenous biogenic amine with a well defined neurotransmitter function in many tissues of the body including the eye [Zifa and Fillion, Pharmacol. Rev., 44:401–458, 1992; Hoyer et al., Pharmacol. Rev., 46:157–203, 1994; Tobin et al., J. Neurosci., 8:3713–3721, 1988].

5HT can interact with at least seven major 5HT receptors (5HT₁–5HT₇) and additional subtypes within these families to initiate intracellular biochemical events such as stimulation of second messengers (e.g. cAMP, inositol trisphosphate) eventually leading to the final biological response, for example, tissue contraction or hormone release, etc. [Hoyer et al., supra; Martin et al., Trends Pharmacol. Sci., 19:2–4, 1998]. Receptor subtypes within the 5HT₁ family are negatively coupled to adenylyl cyclase (AC) and cause inhibition of cAMP production, while 5HT₄, 5HT₆, and 5HT₇ receptors are positively coupled to AC and thus stimulate cAMP production when activated by 5HT [Martin et al., supra]. The receptors in the 5HT₂ family are positively coupled to phospholipase C (PLC) and thus generate inositol phosphates and mobilize intracellular calcium when activated to mediate the effects of 5HT. The 5HT₃ receptor is unique in that it couples to an ion channel which gates sodium, potassium, and calcium [Hoyer et al., supra].

The human and animal 5HT₇ receptor has only recently been cloned, expressed, and shown to be present in various brain areas and peripheral tissues [Eglen et al., Trend Pharmacol. Sci., 18:104–107, 1997]. Recent studies have shown there to be four splice variants of the 5HT₇ receptor [Heidmann et al., J. Neurochem., 68:1372–1381, 1997]. It has been proposed that the 5HT₇ receptor may be involved in the pathophysiology of sleep disorders, depression, and other psychiatric disorders [Eglen et al., supra]. In the periphery, stimulation of 5HT₇ receptors results in relaxation of blood vessels and hence vasodilation [Eglen et al., supra]. Improving blood flow to the back of the eye, including the retina, the macula, and the optic nerve head is believed to be beneficial in the treatment of a number of retinal diseases, for example, glaucoma, ARMD, and diabetic retinopathy [Chiou, et al., J. Ocular Pharmacol. 9:13–24 (1993)].

Serotonergic nerves innervate the eye [Tobin et al., J. Neurosci., 8:3713–3721, 1988] and 5HT has been found in the aqueous humor of human eyes [Martin et al., Ophthalmol., 95:1221–1226, 1988]. In addition, receptor binding sites for [³H]5HT have been demonstrated and pharmacologically characterized in the iris-ciliary body (ICB) of rabbits [Mallorga and Sugrue, Curr. Eye Res., 6:527–532, 1987 and Chidlow et al., Invest. Ophthalmol. Vis. Sci., 36:2238–2245, 1995]. These 5HT binding sites have been shown to be functionally coupled to second messenger generation in rabbits [Tobin and Osborne, J. Neurochem., 53:686–601, 1989 and Tobin et al., J. Neurosci, supra]. In the human ICB these binding sites are characterized as 5HT_(1A) and 5HT₂ receptors [Barnet and Osborne, Exp. Eye Res., 57:209–216, 1993]. In addition, the presence of mRNAs for 5HT_(1a) and 5HT₇ receptors in the rabbit ICB have been reported [Chidlow et al., Invest. Ophthalmol. Vis. Sci., supra and Osborne and Chidlow, Ophthalmologica, 210:308–314, 1996]. The precise functions of these receptors in the eye are unknown, especially the 5HT₇ subtype(s).

5HT or 5-carboxamidotryptamine (5-CT) topically applied to the rabbit eye raise intraocular pressure in the anterior chamber of the eye [Meyer-Bothling et al., Invest. Ophthalmol. Vis. Sci., 34:3035–3042, 1993]. By contrast, it has been shown that topically applied 5HT lowers IOP [Krootila et al., J. Ocular Pharmacol., 3:279–290, 1987 (intracamerally 5HT raised IOP and caused breakdown of the blood-aqueous barrier)]. In addition, the 5HT uptake inhibitor, fluoxetine (Prozac®), also raises IOP in human subjects upon oral administration [Costagliola et al., Br. J. Ophthalmol., 80:678, 1996] and may cause glaucoma [Ahmad, Ann. Pharmacother., 25:436, 1992]. However, the 5HT receptor subtype(s) involved in the IOP-elevating effects of 5HT, 5-CT and fluoxetine are unknown.

Studies conducted in rabbits with 8-hydroxy DPAT and MKC-242 (5HT_(1A) agonists) have shown these compounds lower IOP [Osborne and Chidlow, Ophthalmologica, 210:308–319, 1996, and EP 0771563-A2]. In addition, 5-methylurapidil (5HT_(1A) agonist) lowered IOP in glaucomatous monkeys [Wang, et al., Curr. Eye Res., 16:679–775, 1997]. Both MKC-242 and 5-methylurapidil are relatively potent α1 receptor antagonists (α1 antagonists are known to lower IOP in rabbits, monkeys, and man). The mechanism of action for lowering IOP by 5-methylurapidil has been attributed to its α1 antagonist activity and not its 5HT_(1A) agonist activity [Wang, et al., Invest. Ophthal. Vis. Sci., 39(Suppl):2236–488, 1998]. U.S. Pat. No. 5,693,654, discloses 5HT₁ receptor agonists for lowering IOP. WO92/20333 discloses certain 5HT_(1A) agonists for the treatment of glaucoma.

Methysergide (5HT₂ antagonist) lowered IOP in rabbits [Krootila, et al., Esp. Eye Res., supra]. Ketanserin (5HT_(2A/C) antagonist), also with significant α1 antagonist activity, lowers IOP in rabbits and man [Chan, et al., J. Ocular Pharmacol., 1:137–147, 1985 and Costagliola, et al., Ex. Eye Res., 52:507–510, 1991]. Saprogrelate (5HT_(2A) antagonist) lowers IOP in rabbits and in man when dosed topically or orally [Mano, et al., Invest. Ophthal. Vis. Sci., 36(Suppl):3322–309, 1995, and Takenaka, et al., Invest Ophthal. Vis. Sci., 36(Suppl):3390–377, 1995]. EP 522226 and U.S. Pat. No. 5,290,781 disclose the use of ketanserin and its derivatives for treating ocular hypertension. U.S. Pat. Nos. 5,290,781 and 5,106,555 discloses the use of certain 5HT₂ antagonists for lowering IOP. U.S. Pat. No. 5,652,272 discloses saprogrelate for reducing IOP. U.S. Pat. No. 5,538,974 discloses opthalmic compositions of certain 5HT₂ antagonists for lowering IOP.

U.S. Pat. No. 5,011,846 discloses certain 5HT₃ receptor antagonists for treating glaucoma.

WO 97/17345 discloses that particular compounds with 5HT₄ serotonergic receptor agonist or antagonist activity are useful for treating psychiatric, gastrointestinal, lower urinary, and cardiovascular disorders. The publication mentions the compounds may also be useful for glaucoma.

As evidenced by the previous discussion, it is not clear which serotonergic receptor activity is responsible for lowering IOP. Moreover, a number of these compounds are known to have activity at other receptors which are known to be involved in lowering IOP. Furthermore, it has not been cleared which receptor(s) might be responsible for increasing blood flow and providing neuroprotection in the eye.

SUMMARY OF THE INVENTION

The present invention is directed to Compounds, some of which are novel, that have 5HT₇ receptor affinity, and the use of compounds with 5HT₇ receptor affinity to lower IOP, improve blood flow to the optic nerve head and the retina, provide neuroprotection, and control damage associated with diseases, such as, glaucoma, ARMD, optic neuritis, ischemic disorders, and retinal edema by functioning as neuroprotectants. Compositions of the compounds are contemplated for such uses. The Compounds are also useful for treating sleep disorders, depression, and other psychiatric disorders, such as, schizophrenia, anxiety, obsessive compulsive disorder, circadian rhythm disorders, and centrally and peripherally mediated hypertension.

DETAILED DESCRIPTION PREFERRED EMBODIMENTS

It has been unexpectedly discovered that 5HT₇ receptors are present in the retina, choroid, and possibly the optic nerve head. Furthermore, sertonergic Compounds which possess a relatively high affinity (K_(i)=0.01−200 nM) for 5HT₇ receptors effectively lower elevated IOP. It is believed that these Compounds can improve blood flow, and provide neuroprotection to the optic nerve head and the retina. The Compounds' (preferrably Compounds that are agonists or partial agonists) ability to improve blood flow to the optic nerve head and the retina and other characteristics are believed to render them neuroprotective. The novel Compounds disclosed herein are also useful for treating sleep disorders, depression, and other psychiatric disorders.

Compounds found in the following applications are useful according to the present invention and are incorporated herein by reference: EP 738513-A1; WO 97/29097; WO 97/48681; WO 97/49695; and WO 98/00400. Specific Compounds include: LY-215840, SB-258719, and DR-4004.

The following novel Compounds and their pharmaceutically acceptable salts and solvates are useful for treating persons with the diseases and disorders previously described.

Wherein the dashed bond represents a single or double bond;

-   Aryl signifies a fused phenyl or monocyclic heteroaromatic ring; -   R¹ is H, OH, OC₁₋₃alkyl, C₁₋₃alkyl, C₁₋₃alkyl substituted optionally     with OH, or OC₁₋₃alkyl; -   R² is H, halogen, C₁₋₃alkyl, CONR⁵R⁶, S(═O)_(m)C₁₋₃alkyl, S(═O)₂     NR⁵R⁶, C₁₋₃alkyl substituted optionally with OH, or OC₁₋₃alkyl; -   R³, R⁴ are independently H, C₁₋₃alkyl, C₁₋₃alkyl substituted     optionally with OH or OC₁₋₃alkyl; -   R⁵, R⁶ are independently H, C₁₋₃alkyl, C₂₋₃alkyl substituted     optionally with OH, OC₁₋₃alkyl, or R⁵ and R⁶ can be joined together     with saturated carbon atoms to form a 5 or 6 membered ring and said     carbon atoms can be either unsubstituted or substituted optionally     with C₁₋₃alkyl, C₂₋₃alkyl substituted optionally with OH or     OC₁₋₃alkyl; -   R⁷, R⁸ are together with the nitrogen atom to which they are     attached incorporated into a heterocyclic ring of 5 to 8 atoms which     may include a second heteroatom selected from N, O, S, such as     pyrrolidine, piperidine, Δ³-piperidein, piperazine, morpholine or     thiomorpholine which can be unsubstituted or substituted on carbon     with one or more substituents optionally selected from C₁₋₃alkyl,     C₁₋₃alkyl substituted optionally with OH, OC₁₋₃alkyl, phenyl which     can be unsubstituted or substituted optionally with halogen, CF₃,     OC₁₋₃alkyl, or C₁₋₃alkyl, or substituted on nitrogen with C₁₋₄alkoxy     or phenyl which can be unsubstituted or substituted optionally with     halogen, CF₃, OC₁₋₃alkyl, or C₁₋₃alkyl; -   n is 2 to 4; -   m is 0, 1 or 2.

Wherein the dashed bond represents a single or double bond;

-   Aryl signifies a fused phenyl or monocyclic heteroaromatic ring; -   R¹ is H, C₁₋₅alkyl, C₃₋₅alkenyl, an aromatic ring such as phenyl,     thienyl, pyridyl, and imidazoyl which is either unsubstituted or     substituted optionally with OH, OC₁₋₃alkyl, S(═O)_(m)C₁₋₃alkyl,     halogen, CF₃, or S(═O)₂ NR⁵R⁶; or C₂₋₅alkyl substituted optionally     with OH, OC₁₋₃alkyl, S(═O)_(m)C₁₋₃alkyl or an aromatic ring such as     phenyl, thienyl, pyridyl, and imidazoyl which is either     unsubstituted or substituted optionally with OH, OC₁₋₃alkyl,     S(═O)_(m)C₁₋₃alkyl, halogen, CF₃, S(═O)₂ NR⁵R⁶; or C₃₋₅alkenyl     substituted optionally with OH, OC₁₋₃alkyl, or S(═O)_(m)C₁₋₃alkyl; -   R² is H, halogen, C₁₋₃alkyl, S(═O)_(m)C₁₋₃alkyl, S(═O)₂ NR⁵R⁶, or     C₁₋₃alkyl substituted optionally with OH, or OC₁₋₃alkyl; -   R³ & R⁴ are independently H, C₁₋₃alkyl, or C₁₋₃alkyl substituted     optionally with OH or OC₁₋₃alkyl; -   R⁵, R⁶ are independently H, C₁₋₃alkyl, C₂₋₃alkyl substituted     optionally with OH, OC₁₋₃alkyl, or R⁵ and R⁶ can be joined together     with saturated carbon atoms to form a 5 or 6 membered ring and said     carbon atoms can be either unsubstituted or substituted optionally     with C₁₋₃alkyl, C₂₋₃alkyl substituted optionally with OH or     OC₁₋₃alkyl; -   R⁷, R⁸ are together with the nitrogen atom to which they are     attached incorporated into a heterocyclic ring of 5 to 8 atoms which     may include a second heteroatom selected from N, O, S, such as     pyrrolidine, piperidine, Δ³-piperidein, piperazine, morpholine or     thiomorpholine which can be unsubstituted or substituted on carbon     with one or more substituents optionally selected from C₁₋₃alkyl,     C₁₋₃alkyl substituted optionally with OH, OC₁₋₃alkyl, phenyl which     can be unsubstituted or substituted optionally with halogen, CF₃,     OC₁₋₃alkyl, or C₁₋₃alkyl, or substituted on nitrogen with C₁₋₄alkoxy     or phenyl which can be unsubstituted or substituted optionally with     halogen, CF₃, OC₁₋₃alkyl, or C₁₋₃alkyl; -   n is 2 to 4; -   m is 0, 1 or 2.

-   R³ & R⁴ are independently H, C₁₋₃alkyl, or C₁₋₃alkyl substituted     optionally with OH or OC₁₋₃alkyl; -   R⁷, R⁸ are together with the nitrogen atom to which they are     attached incorporated into a heterocyclic ring of 5 to 8 atoms which     may include a second heteroatom selected from N, O, S, such as     pyrrolidine, piperidine, Δ³-piperidein, piperazine, morpholine or     thiomorpholine which can be unsubstituted or substituted on carbon     with one or more substituents optionally selected from C₁₋₃alkyl,     C₁₋₃alkyl substituted optionally with OH, OC₁₋₃alkyl, phenyl which     can be unsubstituted or substituted optionally with halogen, CF₃,     OC₁₋₃alkyl, or C₁₋₃alkyl, or substituted on nitrogen with C₁₋₄alkoxy     or phenyl which can be unsubstituted or substituted optionally with     halogen, CF₃, OC₁₋₃alkyl, or C₁₋₃alkyl; -   R⁹ is phenyl or a monocyclic heteroaromatic ring which can be     unsubstituted or substituted with C₁₋₄ alkyl, halogen, OC₁₋₄alkyl; -   R¹⁰ is C₁₋₄alkyl, or R¹⁰ can be joined to R⁹ to form a fused     bicyclic ring system such as indoline; -   n is 2 to 4.

-   R³ & R⁴ are independently H, C₁₋₃alkyl, or C₁₋₃alkyl substituted     optionally with OH or OC₁₋₃alkyl; -   R⁷, R⁸ are together with the nitrogen atom to which they are     attached incorporated into a heterocyclic ring of 5 to 8 atoms which     may include a second heteroatom selected from N, O, S, such as     pyrrolidine, piperidine, Δ³-piperidein, piperazine, morpholine or     thiomorpholine which can be unsubstituted or substituted on carbon     with one or more substituents optionally selected from C₁₋₃alkyl,     C₁₋₃alkyl substituted optionally with OH, OC₁₋₃alkyl, phenyl which     can be unsubstituted or substituted optionally with halogen, CF₃,     OC₁₋₃alkyl, or C₁₋₃alkyl, or substituted on nitrogen with C₁₋₄alkoxy     or phenyl which can be unsubstituted or substituted optionally with     halogen, CF₃, OC₁₋₃alkyl, or C₁₋₃alkyl; -   R¹¹ is C₁₋₃alkyl, phenyl or a monocyclic heteroaromatic ring which     can be unsubstituted or substituted with C₁₋₄ alkyl, halogen,     OC₁₋₄alkyl; -   R¹² is C₁₋₄alkyl or a fused bicyclic heteroaromatic ring such as     thieno[3,2-e]-1,2-thiazine, or 1,2-benzothiazine, or R¹² can be     joined to R¹¹ to form a fused bicyclic ring system such as     2,3-dihydro-benzo[c]isoxazole; -   n is 2 to 4.

The compounds of the present invention can be prepared using chemical synthesis procedures herein described. The preferred method for preparing compounds of Formula I is illustrated in Scheme I. For example, the thiazine alcohols 1, which can be prepared by methods described in U.S. Pat. Nos. 5,344,929 and 5,470,973, or in J. Org. Chem. 31, 162 (1966), can be selectively alkylated on the nitrogen atom at position two with, for example, a dihaloalkane using procedures known to the art to give 2, where X is a halogen atom such as chlorine, bromine, or iodine. Compounds 2 can be treated with amines by known procedures to provide compounds of Formula I (3) where R¹ is hydroxyl, further these alcohols 3 can be treated with an alkylhalide to effect alkylation on oxygen to provide the ethers, R¹ is alkoxy. Alternately, 2 can be dehydrated by using methods described in U.S. Pat. No. 5,538,966 to give compounds 4 which can be further reacted with amines to give compounds of Formula I where R¹ is hydrogen and the thiazine ring contains a double bond (5).

Procedures for preparing compounds of Formula II are illustrated in Scheme II. For example, the 3-hydroxymethyl thiazine compounds 7 can be prepared from the esters 6 by methods described in U.S. Pat. No. 5,538,966 [Equation (a)]. Further, compounds 7 can be aminated using a variety of well known procedures, such as initial activation of the hydroxyl group by forming a sulfonate ester, followed by reaction of this intermediate with the desired primary or secondary amine to give compounds 8 of Formula II where R³ and R⁴ are hydrogen and n is 1 [Equation (b)]. Additionally, using 7 as an intermediate with which to initiate a suitable homologation sequence, compounds of Formula II wherein R³ and R⁴ are hydrogen and n is 2 or 3 can be prepared; an example of such a homologation sequence employing 7 is illustrated in Equations (c) and (d), respectively.

The preparation of compounds of Formula III can be readily accomplished by procedures herein described. For example, reaction of the desired amine 14 with the appropriate haloalkylsulfonyl chloride 15 in an inert solvent in the presence of a suitable base [see e.g., J. Med. Chem. 40, 3217 (1997)] to give the haloalkylsulfonamide intermediate 16. Subsequent reaction of 16 with the appropriate primary or secondary amine employing known procedures, provides compounds 17 of Formula III.

The preparation of compounds of Formula IV can be readily accomplished by procedures herein described. For example, reaction of the desired primary amine 18 with the appropriate sulfonyl chloride in an inert solvent in the presence of a suitable base provides the intermediate secondary sulfonamide 19 which can be alkylated by known procedures with the appropriately substituted alkyldibromide to give the haloalkylsulfonamide intermediate 20. Subsequent reaction of 20 with the appropriate primary or secondary amine employing well known procedures provides compounds 21 of Formula IV.

It is evident that some of the Compounds of Formula I–IV will include asymmetric atoms, all enantiomers and diastereomers are contemplated.

The term heteroaromatic ring refers to thiophene, furan, pyrrole, pyridine, pyrimidine, pyridazine and pyrazine.

The Compounds can be administered systemically or locally to the eye (e.g., topically, intracamerally, or via an implant). The Compounds are preferrably incorporated into topical ophthalmic formulations for delivery to the eye. The Compounds may be combined with ophthalmologically acceptable preservatives, surfactants, viscosity enhancers, penetration enhancers, buffers, sodium chloride, and water to form an aqueous, sterile ophthalmic suspension or solution. Ophthalmic solution formulations may be prepared by dissolving a Compound in a physiologically acceptable isotonic aqueous buffer. Further, the ophthalmic solution may include an ophthalmologically acceptable surfactant to assist in dissolving the Compound. Furthermore, the ophthalmic solution may contain an agent to increase viscosity, such as, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylmethylcellulose, methylcellulose, polyvinylpyrrolidone, or the like, to improve the retention of the formulation in the conjunctival sac. Gelling agents can also be used, including, but not limited to, gellan and xanthan gum. In order to prepare sterile ophthalmic ointment formulations, the active ingredient is combined with a preservative in an appropriate vehicle, such as, mineral oil, liquid lanolin, or white petrolatum. Sterile ophthalmic gel formulations may be prepared by suspending the active ingredient in a hydrophilic base prepared from the combination of, for example, carbopol-940, or the like, according to the published formulations for analogous ophthalmic preparations; preservatives and tonicity agents can be incorporated. The Compounds can be formulated for systemic (e.g. oral, I.V., I.M., subcutaneous) delivery according to methods known to one skilled in the art. For systemic delivery the Compounds are delivered at concentrations of 0.005–1000 mg. per dose, preferrably 0.05–20.0, most preferrably 0.2–5 mg. per dose. The Compounds will be dosed 1–4 times per day according to the discretion of a skilled clinician.

For ophthalmic medications the Compounds are preferably formulated as topical ophthalmic suspensions or solutions, with a pH of about 5 to 8. The Compounds will normally be contained in these formulations in an amount 0.01% to 5% by weight, but preferably in an amount of 0.25% to 2% by weight. Thus, for topical presentation 1 to 2 drops of these formulations would be delivered to the surface of the eye 1 to 4 times per day according to the routine discretion of a skilled clinician. The preferred Compounds are those set forth in Examples 1, 1.1, 1.2, 1.6, 1.8, 2.3, 2.7, 2.10, 2.1, 2.4, 3, 3.1, 3.11, 3.5, and 3.10.

EXAMPLE 1 6-Chloro-2-[4-[4-(2H-benzimidazo-2-oxo-1-yl)piperidin-1-yl]butyl]-2H-thieno[3,2-e]-1,2-thiazine 1,1-dioxide Hydrochloride

-   Step 1. A solution     6-chloro-3,4-dihydro-2H-thieno[3,2-e]-1,2-thiazine-4-ol 1,1-dioxide     (9.0 g, 37.6 mmol) in dimethylformamide (200 mL, anhydrous) and     sodium hydride (60% in oil, 1.66 g, 41.5 mmol) was reacted with     1,4-dibromobutane at 0°. The reaction was stirred in an ice bath for     30 min and then it was allowed to warm to room temperature and stir     for three days. The mixture was poured into ice water (400 mL) and     extracted with diethyl ether (2×200 mL). The combined organic layers     were washed with water (200 mL), brine (200 mL) and then were dried     over magnesium sulfate and evaporated. The resulting residue was     purified by silica gel flash chromatography with hexane/ethyl     acetate (7:3) to give     6-chloro-3,4-dihydro-2-(4-bromobutyl)-2H-thieno[3,2-e]-1,2-thiazine-4-ol     1,1-dioxide as a colorless oil (10.62 g, 75%); the ¹H NMR was     consistent with the structure. -   Step 2. The product from Step 1 (10.6 g, 28.3 mmol) was dissolved in     tetrahydrofuran (anhydrous, 400 mL) and treated with triethyl amine     (9.88 mL, 70.9 mmol) and methane sulfonic anhydride (9.86 g, 56.6     mmol) at room temperature and stirred for one hour. The suspension     was concentrated and taken up in dimethylformamide (anhydrous, 120     mL). This mixture was heated at 160° for 45 min. The reaction     mixture was poured into ice water (300 ml) and extracted with     dichloromethane (300 mL). The organic layer was washed with water     (2×200 mL), dried over magnesium sulfate and evaporated to a brown     oil. After silica flash chromatography with hexane/ethyl acetate     6-chloro-2-(4-bromobutyl)-2H-thieno[3,2-e]-1,2-thiazine 1,1-dioxide     was obtained as a yellow oil (4.97 g, 49%); the ¹H NMR. was     consistent with the structure. -   Step 3. A solution of 4-(2H-benzimidazo-2-oxo-1-yl)piperidine (0.30     mmol) in DMF (1.6 mL, anhydrous) and triethyl amine (0.5 mL) was     treated with the product of Step 2 (0.103 g, 0.29 mmol) and stirred     at 70° for 20 hours and then at room temperature for two days. The     reaction mixture was diluted with ethyl acetate (3 mL) and water (4     mL). Saturated sodium bicarbonate (1 mL) was added and the layers     were mixed followed by removal of the aqueous layer. The organic     layer was washed with water (6 mL) and evaporated to give a residue     that was dissolved in ethanol and treated with 1 N hydrochloric acid     in ether. After evaporation the desired product was obtained as a     white solid (69.2 mg, 45%): ¹H NMR and MS (M+H 493) were consistent     with the structure.     By following the procedures of Example 1, but replacing     4-(2H-benzimidazo-2-oxo-1-yl)piperidine in Step 3 with the     appropriate amine, the following compounds were prepared. The ¹H NMR     spectrum and the mass spectrum for each of these compounds were     consistent with the assigned structure. -   1.     6-Chloro-2-[4-(4-phenylpiperazin-1-yl)butyl]-2H-thieno[3,2-e]-1,2-thiazine     1,1-dioxide hydrochloride; -   2.     6-Chloro-2-[4-[4-(2-fluorophenyl)piperazin-1-yl]butyl]-2H-thieno[3,2-e]-1,2-thiazine     1,1-dioxide hydrochloride; -   3.     6-Chloro-2-[4-[4-hydroxy-4-(4-chlorophenyl)piperidin-1-yl]butyl]-2H-thieno[3,2-e]-1,2-thiazine     1,1-dioxide hydrochloride; -   4.     6-Chloro-2-[4-[4-hydroxypiperidin-1-yl]butyl]-2H-thieno[3,2-e]-1,2-thiazine     1,1-dioxide hydrochloride.     By following the procedures of Example 1, but replacing the     1,4-dibromobutane in Step 1 with 1,3-dibromopentane and     4-(2H-benzimidazo-2-oxo-1-yl)piperidine in Step 3 with the     appropriate amine, the following compounds were prepared. The ¹H NMR     spectrum and the mass spectrum for each of these compounds were     consistent with the assigned structure. -   5.     6-Chloro-2-[3-[4-phenylpiperazin-1-yl]propyl]-2H-thieno[3,2-e]-1,2-thiazine     1,1-dioxide hydrochloride; -   6.     6-Chloro-2-[3-[4-(3-trifluoromethylphenyl)piperazin-1-yl]propyl]-2H-thieno[3,2-e]-1,2-thiazine     1,1-dioxide hydrochloride; -   7.     6-Chloro-2-[3-[4-(2-fluorophenyl)piperazin-1-yl]propyl]-2H-thieno[3,2-e]-1,2-thiazine     1,1-dioxide hydrochloride; -   8.     6-Chloro-2-[3-[4-(2H-benzimidazol-2-oxo)piperidin-1-yl]propyl]-2H-thieno[3,2-e]-1,2-thiazine     1,1-dioxide hydrochloride.

EXAMPLE 2 3-(4-Methylpiperidin-1-yl)propylsulfonyl-2,3-dihydro-1H-indole Hydrochloride

-   Step 1. To a solution of indoline (4.00 g, 33.6 mmol) in 100 mL of     acetone at 0° C. was added 3-chloropropanesulfonyl chloride (5.95 g,     33.6 mmol) with stirring. A solid precipitated from the solution.     Diisopropylethylamine (4.33 g, 33.6 mmol) was added in two portions     and the reaction mixture became a homogenous solution. The mixture     was stirred for 30 min, warmed to ambient temperature, and     evaporated to dryness. The crude mixture was combined with a     saturated aqueous solution of sodium bicarbonate and extracted with     ethyl acetate (2×100 mL). Chromatography on silica (10% to 25% ethyl     acetate/hexane) gave an oil which solidified on standing (7.68 g,     77%, mp 53–53° C.). -   Step 2. A mixture of the product of Step 1 (200 mg, 0.77 mmol) and     0.5 M solution of 4-methylpiperidine (4 mL, 2.0 mmol) was heated at     3° C. for 60 h. The reaction mixture was combined with a saturated     aqueous solution of sodium bicarbonate and extracted with ethyl     acetate (2×10 mL). The extracts were dried and evaporated to     dryness. The crude product was filtered though a short silica column     and treated with a 1.0 M solution of hydrogen chloride gas in ether.     The solid was filtered and dried to give the hydrochloride salt (220     mg, 80%): MS(ES) 323 (M+H).     By following the procedures of Example 2, but replacing     4-methylpiperidine in Step 2 with the appropriate amine, the     following compounds were prepared. The ¹H NMR spectrum and the mass     spectrum for each of these compounds were consistent with the     assigned structure. -   1.     3-[4-(3-Chlorophenyl)piperazin-1-yl]propylsulfonyl-2,3-dihydro-1H-indole; -   2. 3-(3-Methylpiperidin-1-yl)propylsulfonyl-2,3-dihydro-1H-indole; -   3.     3-(1,2,3,4-Tetrahydroisoquinolin-2-yl)propylsulfonyl-2,3-dihydro-1H-indole; -   4.     3-[4-(3-Trifluoromethylphenyl)piperazin-1-yl]propylsulfonyl-2,3-dihydro-1H-indole; -   5. 3-(4-Phenylpiperazin-1-yl)propylsulfonyl-2,3-dihydro-1H-indole; -   6.     3-[4-(2-Fluorophenyl)piperazin-1-yl]propylsulfonyl-2,3-dihydro-1H-indole; -   7.     3-[4-(2-Methoxyphenyl)piperazin-1-yl]propylsulfonyl-2,3-dihydro-1H-indole; -   8.     3-[4-(4-Methoxyphenyl)piperazin-1-yl]propylsulfonyl-2,3-dihydro-1H-indole; -   9.     3-[4-(2-Chlorophenyl)piperazin-1-yl]propylsulfonyl-2,3-dihydro-1H-indole.     By following the procedures of Example 2, but replacing the indoline     in Step 1 with N-methylaniline and the 4-methylpiperidine in Step 2     with the appropriate amine, the following compounds were prepared.     The ¹H NMR spectrum and the mass spectrum for each of these     compounds were consistent with the assigned structure. -   10.     3-(1,2,3,4-Tetrahydroisoquinolin-2-yl)-N-methyl-N-phenyl-propylsulfonamide; -   11.     N-Methyl-N-phenyl-3-[4-(3-trifluoromethylphenyl)piperazin-1-yl]propylsulfonamide; -   12. N-Methyl-N-phenyl-3-(4-phenylpiperazin-1-yl)propylsulfonamide; -   13.     3-[4-(2-Fluorophenyl)piperazin-1-yl]-N-methyl-N-phenyl-propylsulfonamide; -   14.     N-Methyl-3-[4-(2-methoxyphenyl)piperazin-1-yl]-N-phenyl-propylsulfonamide; -   15.     3-[4-(2-Chlorophenyl)piperazin-1-yl]-N-methyl-N-phenyl-propylsulfonamide     By following the procedures of Example 2, but replacing the     3-chloropropanesulfonyl chloride in Step 1 with     2-chloroethanesulfonyl chloride and the 4-methylpiperidine in Step 2     with 3-methylpiperidine, the following compound was prepared. The ¹H     NMR spectrum and the mass spectrum for this compound were consistent     with the assigned structure. -   16. 2-(3-Methylpiperidin-1-yl)ethylsulfonyl-2,3-dihydro-1H-indole.

EXAMPLE 3 N-[3-[4-(3-Chlorophenyl)piperazin-1-yl]propyl]-N-(4-methoxyphenyl)-propanesulfonamide Hydrochloride

-   Step 1. To a solution of p-anisidine (6.00 g, 48.7 mmol) and     triethylamine (5.91 g, 58.4 mmol) in methylene chloride (200 mL) at     0° C. was added propylsulfonyl chloride (7.64 g, 53.6 mmol) with     stirring under nitrogen atmosphere. The reaction mixture was allowed     to warm to room temperature and stirred overnight. The mixture was     washed with a saturated aqueous solution of sodium bicarbonate (100     mL), water, and dried over magnesium sulfate. The organic layer was     evaporated to give an oil that was mixed with a solution of hexane     and ethyl acetate (3:1) to afford a crystalline solid (7.97 g). The     mother liquid was chromatographed on silica (hexane/ethyl acetate,     4:1) to give a solid (2.27 g, 92%): mp 72° C.; MS(−ES) 228 (M−H). -   Step 2. To the product of Step 1 (3.50 g, 15.3 mmol) in anhydrous     dimethylformamide (80 mL) at 0° C. was added sodium hydride (60%     suspension in mineral oil, 0.672 g, 16.8 mmol) under a nitrogen     atmosphere. The suspension was stirred for 30 min and     1,3-dibromopropane (9.27 g, 45.9 mmol) was added over 1 min. The     reaction was stirred for 3 h, mixed with a saturated aqueous     solution of sodium bicarbonate (200 mL) and extracted with ethyl     acetate (3×100 mL). The combined extracts were dried and evaporated     to dryness. Chromatography on silica (20% ethyl acetate in hexane)     gave a colorless oil (4.33 g, 81%): MS(+ES) 352 (M+H). -   Step 3. To a solution of the product of Step 2 (0.175 g, 0.50 mmol)     in anhydrous dimethylformamide (1 mL) was added a 0.5 M solution of     1-(3-chlorophenyl)piperazine in dimethylformamide (1.1 mL, 0.55     mmol) and triethylamine (0.20 mL); this mixture was heated at 60° C.     for 18 h. The cooled reaction mixture was extracted with ethyl     acetate (2×1 mL) and the combined extracts were washed with a     saturated aqueous solution of sodium bicarbonate, dried and     evaporated to an oil which was treated with a 1.0 M solution of     hydrogen chloride gas in ether to give the corresponding salt (0.11     g, 44%): MS(ES) 466 (M+).     By following the procedures of Example 3, but replacing     1-(3-chlorophenyl)piperazine in Step 3 with the appropriate amine,     the following compounds were prepared. The ¹H NMR spectrum and the     mass spectrum for each of these compounds were consistent with the     assigned structure. -   1.     N-[3-(1,2,3,4-Tetrahydroisoquinolin-2-yl)propyl]-N-(4-methoxyphenyl)-propanesulfonamide; -   2.     N-[3-(3-Hydroxymethylpiperidin-1-yl)propyl]-N-(4-methoxyphenyl)-propanesulfonamide; -   3.     N-(4-Methoxyphenyl)-N-[3-(morpholin-4-yl)propyl]-propanesulfonamide; -   4.     N-(4-Methoxyphenyl)-N-[3-(2-methylpiperidin-1-yl)propyl]-propanesulfonamide; -   5-N-[3-[4-(3-Chlorophenyl)piperazin-1-yl]propyl]-N-(4-methoxyphenyl)-propanesulfonamide; -   6.     N-(4-Methoxyphenyl)-N-[3-[4-(3-trifluoromethylphenyl)piperazin-1-yl]propyl]-propanesulfonamide; -   7.     N-[3-(4-phenylpiperazin-1-yl)propyl]-N-(4-methoxyphenyl)-propanesulfonamide; -   8.     N-[3-[4-(2-Fluorophenyl)piperazin-1-yl]propyl]-N-(4-methoxyphenyl)-propanesulfonamide; -   9.     N-[3-[4-(4-Methoxyphenyl)piperazin-1-yl]propyl]-N-(4-methoxyphenyl)-propanesulfonamide; -   10.     N-[3-[4-(2-Methoxyphenyl)piperazin-1-yl]propyl]-N-(4-methoxyphenyl)-propanesulfonamide; -   11.     N-[3-[4-(2-Chlorophenyl)piperazin-1-yl]propyl]-N-(4-methoxyphenyl)-propanesulfonamide; -   12.     N-[3-[4-(2H-Benzimidazo-2-oxo-1-yl)piperidin-1-yl]propyl]-N-(4-methoxyphenyl)-propanesulfonamide.

By following the procedures of Example 3, but replacing the 1,3-dibromopropane in Step 2 with 1,4-dibromobutane and the 1-(3-chlorophenyl)piperazine in Step 3 with 1,2,3,4-tetrahydroisoquinoline, the following compound was prepared. The ¹H NMR spectrum and the mass spectrum for this compound were consistent with the assigned structure.

-   13.     N-[4-(1,2,3,4-Tetrahydroisoquinolin-2-yl)butyl]-N-(4-methoxyphenyl)-methanesulfonamide.

The following topical ophthalmic formulations are useful according to the present invention administered 1–4 times per day according to the discretion of a skilled clinician.

EXAMPLE 4

Ingredients Amount (wt %) 5HT₇ Compound 0.01–2% Hydroxypropyl methylcellulose  0.5% Dibasic sodium phosphate (anhydrous)  0.2% Sodium chloride  0.5% Disodium EDTA (Edetate disodium) 0.01% Polysorbate 80 0.05% Benzalkonium chloride 0.01% Sodium hydroxide/Hydrochloric acid For adjusting pH to 7.3–7.4 Purified water q.s. to 100%

EXAMPLE 5

Ingredients Amount (wt %) 5HT₇ Compound 0.01–2% Hydroxypropyl methylcellulose  0.5% Cremophor EL  0.1% Tromethamine, USP, AR 0.64% Mannitol, USP  3.0% Boric acid, USP  0.3% Dibasic sodium phosphate (anhydrous)  0.2% Sodium chloride  0.5% Disodium EDTA (Edetate disodium) 0.01% Polysorbate 80 0.05% Benzalkonium chloride 0.01% Sodium hydroxide/Hydrochloric acid For adjusting pH to 7.3–7.4 Purified water q.s. to 100%

EXAMPLE 6

Ingredients Amount (wt %) 5HT₇ Compound 0.01–2% Methyl cellulose  4.0% Dibasic sodium phosphate (anhydrous)  0.2% Sodium chloride  0.5% Disodium EDTA (Edetate disodium) 0.01% Polysorbate 80 0.05% Benzalkonium chloride 0.01% Sodium hydroxide/Hydrochloric acid For adjusting pH to 7.3–7.4 Purified water q.s. to 100%

EXAMPLE 7

Ingredients Amount (wt %) 5HT₇ Compound 0.01–2% Hydroxypropyl-β-cyclodextrin  4.0% Dibasic sodium phosphate (anhydrous)  0.2% Sodium chloride  0.5% Disodium EDTA (Edetate disodium) 0.01% Polysorbate 80 0.05% Benzalkonium chloride 0.01% Sodium hydroxide/Hydrochloric acid For adjusting pH to 7.3–7.4 Purified water q.s. to 100%

EXAMPLE 8

Ingredients Amount (wt %) 5HT₇ Compound 0.01–2% Xanthan gum  0.5–6.0% Dibasic sodium phosphate (anhydrous)  0.2% Sodium chloride  0.5% Disodium EDTA (Edetate disodium) 0.01% Polysorbate 80 0.05% Benzalkonium chloride 0.01% Sodium hydroxide/Hydrochloric acid For adjusting pH to 7.3–7.4 Purified water q.s. to 100%

EXAMPLE 9

Ingredients Amount (wt %) 5HT₇ Compound 0.01–2% Guar gum  0.4–6.0% Dibasic sodium phosphate (anhydrous)  0.2% Sodium chloride  0.5% Disodium EDTA (Edetate disodium) 0.01% Polysorbate 80 0.05% Benzalkonium chloride 0.01% Sodium hydroxide/Hydrochloric acid For adjusting pH to 7.3–7.4 Purified water q.s. to 100%

EXAMPLE 10

Ingredients Amount (wt %) 5HT₇ Compound 0.01–2% Tyloxapol  0.2–4.0% Dibasic sodium phosphate (anhydrous)  0.2% Sodium chloride  0.5% Disodium EDTA (Edetate disodium) 0.01% Polysorbate 80 0.05% Benzalkonium chloride 0.01% Sodium hydroxide/Hydrochloric acid For adjusting pH to 7.3–7.4 Purified water q.s. to 100%

EXAMPLE 11

Ingredients Amount (wt %) 5HT₇ Compound 0.01–2% White petrolatum and mineral oil and Ointment consistency lanolin Dibasic sodium phosphate (anhydrous)  0.2% Sodium chloride  0.5% Disodium EDTA (Edetate disodium) 0.01% Polysorbate 80 0.05% Benzalkonium chloride 0.01% Sodium hydroxide/Hydrochloric acid For adjusting pH to 7.3–7.4

EXAMPLE 12

Formulation for Oral Administration

Tablet: 0.2–5 mg. of 5HT₇ Compound with inactive ingredients such as cornstarch, lactose, colloidal silicon dioxide, microcrystalline cellulose, and magnesium sterate can be formulated according to procedures known to those skilled in the art of tablet formulation. 

1. A compound of the formula:

R³, R⁴ are independently H, C₁₋₃alkyl, or C₁₋₃alkyl substituted optionally with OH or OC₁₋₃alkyl; R⁷, R⁸ are together with the nitrogen atom to which they are attached incorporated into a heterocyclic ring selected from the group consisting of pyrrolidine, piperidine, Δ³-piperidein, piperazine, morpholine or thiomorpholine which can be unsubstituted or substituted on carbon with one or more substituents optionally selected from C₁₋₃alkyl, C₁₋₃alkyl substituted optionally with OH, OC₁₋₃alkyl, phenyl which can be unsubstituted or substituted optionally with halogen, CF₃, OC₁₋₃alkyl, or C₁₋₃alkyl, or substituted on nitrogen with C₁₋₄alkoxy or phenyl which can be unsubstituted or substituted optionally with halogen, CF₃, OC₁₋₃alkyl, or C₁₋₃alkyl; R⁹ is phenyl or a monocyclic heteroaromatic ring which can be unsubstituted or substituted with C₁₋₄ alkyl, halogen, OC₁₋₄alkyl; R¹⁰ is C₁₋₄alkyl, or R¹⁰ can be joined to R⁹ to form an indoline; n is 2 to 4 or a pharmaceutically acceptable salt thereof.
 2. A method for lowering IOP which comprises administering to a person in need thereof, a composition comprising an effective amount of a compound of the formula:

R³, R⁴ are independently H, C₁₋₃alkyl, or C₁₋₃alkyl substituted optionally with OH or OC₁₋₃alkyl; R⁷, R⁸ are together with the nitrogen atom to which they are attached incorporated into a heterocyclic ring selected from the group consisting of pyrrolidine, piperidine, Δ³-piperidein, piperazine, morpholine or thiomorpholine which can be unsubstituted or substituted on carbon with one or more substituents optionally selected from C₁₋₃alkyl, C₁₋₃alkyl substituted optionally with OH, OC₁₋₃alkyl, phenyl which can be unsubstituted or substituted optionally with halogen, CF₃, OC₁₋₃alkyl, or C₁₋₃alkyl, or substituted on nitrogen with C₁₋₄alkoxy or phenyl which can be unsubstituted or substituted optionally with halogen, CF₃, OC₁₋₃alkyl, or C₁₋₃alkyl; R⁹ is phenyl or a monocyclic heteroaromatic ring which can be unsubstituted or substituted with C₁₋₄ alkyl, halogen, OC₁₋₄alkyl; R¹⁰ is C₁₋₄alkyl, or R¹⁰ can be joined to R⁹ to form an indoline; n is 2 to 4 or a pharmaceutically acceptable salt thereof.
 3. A method for improving blood flow to the optic nerve head and the retina which comprises administering to a person in need thereof, a composition comprising an effective amount of a compound of the formula:

R³, R⁴ are independently H, C₁₋₃alkyl, or C₁₋₃alkyl substituted optionally with OH or OC₁₋₃alkyl; R⁷, R⁸ are together with the nitrogen atom to which they are attached incorporated into a heterocyclic ring selected from the group consisting of pyrrolidine, piperidine, Δ³-piperidein, piperazine, morpholine or thiomorpholine which can be unsubstituted or substituted on carbon with one or more substituents optionally selected from C₁₋₃alkyl, C₁₋₃alkyl substituted optionally with OH, OC₁₋₃alkyl, phenyl which can be unsubstituted or substituted optionally with halogen, CF₃, OC₁₋₃alkyl, or C₁₋₃alkyl, or substituted on nitrogen with C₁₋₄alkoxy or phenyl which can be unsubstituted or substituted optionally with halogen, CF₃, OC₁₋₃alkyl, or C₁₋₃alkyl; R⁹ is phenyl or a monocyclic heteroaromatic ring which can be unsubstituted or substituted with C₁₋₄ alkyl, halogen, OC₁₋₄alkyl; R¹⁰ is C₁₋₄alkyl, or R¹⁰ can be joined to R⁹ to form an indoline; n is 2 to 4 or a pharmaceutically acceptable salt thereof.
 4. A method for treating retinal diseases which comprises administering to a person in need thereof, a composition comprising an effective amount of a compound of the formula:

R³, R⁴ are independently H, C₁₋₃alkyl, or C₁₋₃alkyl substituted optionally with OH or OC₁₋₃alkyl; R⁷, R⁸ are together with the nitrogen atom to which they are attached incorporated into a heterocyclic ring selected from the group consisting of pyrrolidine, piperidine, Δ³-piperidein, piperazine, morpholine or thiomorpholine which can be unsubstituted or substituted on carbon with one or more substituents optionally selected from C₁₋₃alkyl, C₁₋₃alkyl substituted optionally with OH, OC₁₋₃alkyl, phenyl which can be unsubstituted or substituted optionally with halogen, CF₃, OC₁₋₃alkyl, or C₁₋₃alkyl, or substituted on nitrogen with C₁₋₄alkoxy or phenyl which can be unsubstituted or substituted optionally with halogen, CF₃, OC₁₋₃alkyl, or C₁₋₃alkyl; R⁹ is phenyl or a monocyclic heteroaromatic ring which can be unsubstituted or substituted with C₁₋₄ alkyl, halogen, OC₁₋₄alkyl; R¹⁰ is C₁₋₄alkyl, or R¹⁰ can be joined to R⁹ to form an indoline; n is 2 to 4 or a pharmaceutically acceptable salt thereof.
 5. A method for treating persons suffering from a sleeping disorder, depression, schizophrenia, anxiety, obsessive compulsive disorders, circadian rhythm disorders, and centrally and peripherally mediated hypertension which comprises, administering a composition comprising a pharmaceutically effective amount of a compound of the formula:

R³, R⁴ are independently H, C₁₋₃alkyl, or C₁₋₃alkyl substituted optionally with OH or OC₁₋₃alkyl; R⁷, R⁸ are together with the nitrogen atom to which they are attached incorporated into a heterocyclic ring selected from the group consisting of pyrrolidine, piperidine, Δ³-piperidein, piperazine, morpholine or thiomorpholine which can be unsubstituted or substituted on carbon with one or more substituents optionally selected from C₁₋₃alkyl, C₁₋₃alkyl substituted optionally with OH, OC₁₋₃alkyl, phenyl which can be unsubstituted or substituted optionally with halogen, CF₃, OC₁₋₃alkyl, or C₁₋₃alkyl, or substituted on nitrogen with C₁₋₄alkoxy or phenyl which can be unsubstituted or substituted optionally with halogen, CF₃, OC₁₋₃alkyl, or C₁₋₃alkyl; R⁹ is phenyl or a monocyclic heteroaromatic ring which can be unsubstituted or substituted with C₁₋₄ alkyl, halogen, OC₁₋₄alkyl; R¹⁰ is C₁₋₄alkyl, or R¹⁰ can be joined to R⁹ to form an indoline; n is 2 to 4 or a pharmaceutically acceptable salt thereof.
 6. A composition comprising a pharmaceutically effective amount of a compound of the formula:

R³, R⁴ are independently H, C₁₋₃alkyl, or C₁₋₃alkyl substituted optionally with OH or OC₁₋₃alkyl; R⁷, R⁸ are together with the nitrogen atom to which they are attached incorporated into a heterocyclic ring selected from the group consisting of pyrrolidine, piperidine, Δ³-piperidein, piperazine, morpholine or thiomorpholine which can be unsubstituted or substituted on carbon with one or more substituents optionally selected from C₁₋₃alkyl, C₁₋₃alkyl substituted optionally with OH, OC₁₋₃alkyl, phenyl which can be unsubstituted or substituted optionally with halogen, CF₃, OC₁₋₃alkyl, or C₁₋₃alkyl, or substituted on nitrogen with C₁₋₄alkoxy or phenyl which can be unsubstituted or substituted optionally with halogen, CF₃, OC₁₋₃alkyl, or C₁₋₃alkyl; R⁹ is phenyl or a monocyclic heteroaromatic ring which can be unsubstituted or substituted with C₁₋₄alkyl, halogen, OC₁₋₄alkyl; R¹⁰ is C₁₋₄alkyl, or R¹⁰ can be joined to R⁹ to form an indoline; n is 2 to 4 or a pharmaceutically acceptable salt thereof in a pharmaceutically acceptable carrier.
 7. The Compound of claim 1 selected from the group consisting of: 3-[4-(3-Chlorophenyl)piperazin-1-yl]propylsulfonyl-2,3-dihydro-1H-indole; 3-[4-(3-Trifluoromethylphenyl)piperazin-1-yl]propylsulfonyl-2,3-dihydro-1H-indole; 3-[4-(2-Methoxyphenyl)piperazin-1-yl]propylsulfonyl-2,3-dihydro-1H-indole. 